Quick and Easy Assessment Strategies For every assignment I do with my students, we must have a list of success criteria. These criteria must be co-created by the students and myself I can "guide" them to include criteria I want in the assignment, but they must feel that they have ownership over the list. Because they are so invested in setting up the criteria, they are more able to accurately assess their own and other's work before handing in for teacher assessment.
This last cycle involved a FET of a day 5 and day 6 blastocyst from an earlier IVF that we were not able to complete a transfer for.
The embryos had been vitrified and when defrosted, we were told they did not expand, and remained shrunk. The embryologist said there were a few cells in there that were still alive but their assessment was that these were not viable.
I did find a study that had compared transfer of blastocysts that had expanded and those that did not, and the pregnancy rate among those that did not was zero.
Thanks for considering this as a topic. In speaking with other embryologists over the years, the majority or programs will as a matter of policy pick the embryos for transfer that scored the highest among the still living embryos.
It is routine for some embryos to stop growing between fertilization and the time of transfer, probably due to genetic issues but not always. So, the remaining non-transferred embryos may have slightly lower quality based on having scored lower-assuming scoring is a good sign of quality—which is not always true.
These possibly second tier embryos are frozen. Of course, if you have a large number of good quality embryos, you will be freezing high quality embryos too. The reason I say this is to explain that not all embryos start off equal at the time of freezing. Embryo quality is not improved by the cryopreservation process; the best outcome is no loss in viability.
The method used to cryopreserve the embryos makes a difference too in the quality and hence the scores of embryos post-thaw. In the bad old days, we used slow freezing which was relatively poor in preserving embryo quality.
If better than half the cells in the embryo appeared clear and shiny after thaw- and thus alive- we called it a good outcome. Now, with vitrification, we expect to see the embryo reappear from the thaw looking as good as it did going in.
The embryo needs to be ready to implant and the uterus needs to be ready to receive the embryo. Clinical staff will use test results from blood work and ultrasound visualization of the uterine lining to adjust medications and decide when the patient is ready for transfer.
For instance, if the patient has embryos frozen at the zygote fertilized egg stagethe embryo will have to be thawed several days in advance of the actual transfer to allow the embryo to continue to grow to the 8 cell stage for a day 3 transfer or the blastocyst stage for a day 5 transfer.
If the embryo was frozen as a blastocyst stage embryo, it will be thawed close to the time of transfer, at most, the afternoon before but most likely the morning of the transfer day.
This naming convention probably arises from the fact that the process of vitrification bypasses ice crystal formation, moving the embryo right into a solid phase- glass-like -state. Ice thaws but glassified embryos warm from a colder state to a body temperature state.
All storage systems have some method of identifying embryos that is physically adhered to the smallest storage unit- the straw or the vial.
Often, a waterproof permanent marker is used to print patient name or and ID number or both with date of freeze or other info on the straw or vial. Newer methods use bar codes. In any case, the right straw is recovered from the cryostorage tank.Fourth Grade Assessments and Scoring Checklists, Common Core State Standards Contents:!
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